A rapid and efficient protocol for the large-scale propagation of a potential medicinal plant, Plumbago zeylanica L., through in vitro culture of nodal explants is described. High frequency multiple shoots were induced on Murashige and Skoog's basal medium supplemented with 3.0 mg/l kinetin (KIN) and 0.5 mg/l 6-benzyl amino purine (BAP). The in vitro developed shoots were rooted best on half-strength MS medium fortified with 1.0 mg/l indole-3-acetic acid (IAA) and 0.5 mg/l 1-naphtalene acetic acid (NAA). The developed micropropagation protocol can be successfully used for large-scale multiplication and conservation of P. zeylanica.


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