Isolation and Identification of a Fungus Infecting Egyptian wheat grains Gamiza11 Triticum aestivum

Anwaar FAYED; Osama M. ABONAMA; Hoda MAHROUS; Ahmed TAYEL

Vol 5 No 1 (2019) / 1-4 Research articles

Isolation and Identification of a Fungus Infecting Egyptian wheat grains Gamiza11 Triticum aestivum

Anwaar FAYED

Osama M. ABONAMA

Hoda MAHROUS

Ahmed TAYEL

More author's info

Anwaar FAYED
Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

Osama M. ABONAMA
Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

Hoda MAHROUS
Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

pvphuc@bmrat.org

Ahmed TAYEL
Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt

Submitted: Sep 26, 2021 | Published: Mar 31, 2019

Vol 5 No 1 (2019) | | Page No.: 1-4

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FAYED, A., M. ABONAMA, O., MAHROUS, H., & TAYEL, A. (2019). Isolation and Identification of a Fungus Infecting Egyptian wheat grains Gamiza11 Triticum aestivum. Biotechnological Research, 5(1), 1-4. Retrieved from http://br.biomedpress.org/index.php/br/article/view/778

Copyrights: Anwaar FAYED, Osama M. ABONAMA, Hoda MAHROUS, Ahmed TAYEL, 2019. License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The Penicillium species are among the most commonly occurring and economically important members of micro-fungi family. In this study, Molecular identification of fungal isolates were used to group and determine genetic variability of Penicillium isolate from Egyptian Gamiza11 wheat grains Triticum aestivum were obtained from Agricultural Administration in Menouf, Governorate of Menoufia, Egypt. The fungal DNA was then sent to SolGent Company, Daejeon South Korea for polymerase chain reaction (PCR) and gene sequencing. PCR was performed using two universal fungal primers ITS1 (forward) and ITS4 (reverse) which were incorporated in the reaction mixture. Molecular analysis also indicated genotype variability between the isolates with little correlation with either the origin of soil or geographical location.