Silver nanoparticles are known to be highly toxic to microorganism showing strong biocidal effect and are nontoxic to human body at low concentration. The aim of this work was to determine the potential of biosynthesized silver nanoparticle’s (BioAgNP’s) antimicrobial activity against food borne pathogen Aeromonas sp. and in water purification. The Aeromonas sp. was isolated from decayed fish. Psidium gaujava plant extract showed high antibacterial activity against the isolated organism. The chemical constituents of the leaf extract were analyzed by Fourier Transform Infrared Spectroscopy (FTIR). Silver nanoparticles were biosynthesized using the P. gaujava plant extract. The characterization of BioAgNP was done by UV–Vis spectroscopy and Scanning Electron Microscopy (SEM). The biosynthesized silver nanoparticle was then adsorbed on Granular Activated Charcoal (GAC) and their presence was confirmed using FTIR. GAC can be used as a bacterial filter for treating contaminated water. The antibacterial action of silver nanoparticle was studied using Colony Forming Unit (CFU) and Most Probable Number (MPN) test. Enumeration of coliforms and percentage of reduction was studied which showed that effectiveness increases with increase in dose of adsorbed charcoal and treatment time. The obtained result showed that the silver adsorbed activated charcoal can be used as excellent antibacterial media and would have several applications in water treatment system.


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