Fifty two lactic acid bacteria (LAB) were isolated from milk of Desi and cross-breed cows of India and its functional characteristics were investigated on the basis of morphological and biochemical properties. Among them four bacterial isolates were screened on the basis of in vitro probiotic attributes. Isolate CP-12d and CP-8d which were isolated from Desi Indian cows showed high tolerance to low pH, bile, NaCl and certain antibiotics. These isolates exhibited the highest adhesion to hydrocarbons xylene, n-octane and n-hectane. Isolate CP-8d exhibited the highest auto-aggregation rate (62%). It was the most resistant isolate against different antibiotics. All the four isolates inhibited the enteric pathogens viz., Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Salmonella typhi but the isolate CP-12d and CP-8d exhibited high antagonistic activity against all pathogens. These two bacterial isolates exhibited good probiotic properties and also grew well in presence of inulin as compared to honey.


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