An efficient and reproducible protocol for in vitro regeneration and flowering of Tridax procumbens was established through nodal explants. Multiple shoot proliferation was observed on MS medium supplemented with 1.0 mg l-1 BAP. High frequency rhizogenesis was observed on half-strength MS medium augmented with 0.5 mg l-1 IBA. In vitro flowering was initiated on medium having BAP (1.0 mg/L) and adenine sulfate (100 mg/L). The present investigation describes hormonal regulation of morphogenesis in vitro in nodal explants of T. procumbens. The study has also laid a preliminary foundation for a further research to understand the complex mechanism of floral development.


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