Various concentrations of sodium alginate and calcium chloride were tested in order to optimize the shape, and texture of synthetic seeds developed through the encapsulation of Protocorm Like Bodies (PLBs) of Asparagus racemosus. Sodium alginate (2.5%) and CaCl2.2H2O (100 mM) solutions were found to be the optimal concentration for production of firm, transparent and uniform synthetic seeds. The beads showed 94.9% germination on MS basal medium within three weeks. The germinated seedlings formed complete plantlet having well developed root-shoot system. The developed protocol for synthetic seed production through PLBs can be used for conservation of A. racemosus.


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