Assessment of Genetic Diversity in Groundnut (Arachis hypogaea L.) Genotypes using PCR based RAPD markers
Cultivated groundnut or peanut is one of the important legume crops of tropical and semiarid tropical countries, where it provides a major source of edible oil and vegetable protein. Knowledge of genetic diversity within and among varieties in a crop is prerequisite to its improvement. The objective of this study is to estimate the genetic diversity and to evaluate the relationship between eight groundnut genotypes using Random Amplified Polymorphic DNA (RAPD). Eight groundnut genotypes were included in this study and were subjected to RAPD analysis. Total 10 primers were used to detect polymorphism but only 8 primers showed amplification. Selected primers generated 52 amplification products, out of which 34 were polymorphic (65.38%). The number of fragments amplified per primers ranged from 2 to 15 and their sizes ranged from ~ 500bp to ~2000bp. Maximum number of amplification products were observed with OPD-02 primer (100% polymorphism). UPGMA cluster analysis among the genotypes revealed that the genotypes Narayani and Dharani are genetically similar with 86.8% whereas Kadiri-6 and Dharani were distant. Genotype specific amplification profiles observed with specific primers would help in the identification of the genotypes resistant to biotic, abiotic stress and agronomically important characters. The alleles represented in 8 groundnut genotypes with 5 RAPD markers and their uniqueness has to be verified over a wide range of genotypes.
Volume & Issue : Vol 2 No 4 (2016)
Page No.: 142-147
Published on: 2016-12-31
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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