Abstract

Micronuclei (MN) assay is an ideal monitoring system that uses aquatic organisms to assess the genotoxicity of water pollutants. The aim of this study was to determine the genotoxic potential of environmentally relevant concentrations of C.I. Reactive Red 120 (RR 120) azo dye on Catla catla, important edible freshwater fingerlings, using DNA damage in gill cells and haemocytes as sensitive biomarkers. It was also about to determine the dose and time dependent induction of frequencies of micronuclei in different tissues examined. For this, fingerlings were exposed to various concentrations of C.I. RR 120 azo dye (10, 20, 30 and 40 ppm). Samples (gills and peripheral blood) were collected and analyzed to check the effect of textile azo dye C.I. RR 120 result suggest that frequency of micronuclei at exposed fingerlings was higher compared with control group. A comparison between micronuclei frequencies in gills cells and haemocytes showed that the highest micronucleus frequency detect in gills cells followed by haemocytes cells. Induction of micronuclei was dose dependent and also time dependent.Results from this study recommend the use of the micronucleus test in fish tissues as a sensitive genotoxic monitor for aquatic pollution. It also enlightens the genotoxic effects of azo dye on various targeted samples by MN test.



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