Abstract

Objectives: Antibacterial activity of bio synthetic silver nanoparticles against Escherichia coli (E. coli) ATCC 8739 and Salmonella typhimurium (S.typhi) ATCC 14028 using Moringa oleifera leaves extract was investigated. Materials and Methods: Prepared silver nanoparticles were characterized by using different techniques included: Ultraviolet Visible Spectroscopy (UV-VIS spectra analysis), Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscope (TEM). The comparison between the antibacterial activities of the concentration 100 µg/ml of bio synthetic silver nanoparticles using Moringa oleifera leaves extract and 100 µg/ml of silver nitrate solution tested against E. coli reference strain ATCC 8739 and S. typhi ATCC 14028 was evaluated by using agar diffusion assay method. Results: FTIR analysis was showed the reduction action of Moringa oleifera leaves extract to form Silver Nanoparticles. TEM shows a dispersion of nanoparticles in a range (15.22 nm – 29.45) by using Moringa oleifera leaves extract. The antimicrobial activity of the concentration 100 µg/ml of synthetized silver nanoparticles was approved against E. coli reference strain ATCC 8739 by showing zone of inhibition against E. coli equal to 18 mm, while 100 µg/ml of the silver nitrate solution showed zone of inhibition against E. coli equal to 17 mm. While The antimicrobial activity of the concentration 100 µg/ml of synthetized silver nanoparticles which tested against S.ty phi reference strain ATCC 14028 by showing showed zone of inhibition against equal to 21 mm, while 100 µg/ml of the silver nitrate solution showed zone of inhibition against S.typhi equal to 15mm