Isolation and Identification of a Fungus Infecting Egyptian wheat grains Gamiza11 Triticum aestivum
- Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt
- Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt
Abstract
The Penicillium species are among the most commonly occurring and economically important members of micro-fungi family. In this study, Molecular identification of fungal isolates were used to group and determine genetic variability of Penicillium isolate from Egyptian Gamiza11 wheat grains Triticum aestivum were obtained from Agricultural Administration in Menouf, Governorate of Menoufia, Egypt. The fungal DNA was then sent to SolGent Company, Daejeon South Korea for polymerase chain reaction (PCR) and gene sequencing. PCR was performed using two universal fungal primers ITS1 (forward) and ITS4 (reverse) which were incorporated in the reaction mixture. Molecular analysis also indicated genotype variability between the isolates with little correlation with either the origin of soil or geographical location.