Comparative Assessment of the Antioxidant Activities, Total Phenolics and Fatty Acid Composition in Three Indian Oats Varieties
- Pharmacology and Toxicology Division, CSIR-Indian Institute of Chemical Technology (IICT), Hyderabad-500 007, India
- Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi- 110 001, India
- ICAR-Indian Grassland and Fodder Research Institute, Regional Research Station, Srinagar, 190007, India
- Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology (IICT), Hyderabad-500 007, India
Abstract
A comparative assessment of the total phenolic content and antioxidant activity of polar extracts of three Indian varieties (SKO 90, Sabzaar and SKO 96) of oats (Avena sativa L.) was carried out. Acidified aqueous ethanolic extracts of oats were sequentially extracted with hexane, ethyl acetate, n-butanol and water to obtain four fractions in each variety. In all varieties, weights of different fractions followed the order: Hexane>water>n-butanol>ethyl acetate. The hexane fraction of all the three varieties was analyzed for lipid composition by gas chromatography. All fractions were evaluated for total phenolic content by the Folin−Ciocalteu method and antioxidant activity using three in vitro assays, namely DPPH, ABTS and Nitric Oxide scavenging assays. Quantitative estimation of fatty acids in the hexane-soluble fraction of all the three varieties demonstrated that all the varieties had similar fatty acid composition. However, the antioxidant assays demonstrated that the SKO 90 variety had better antioxidant potential followed by SKO 96 and Sabzaar.