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Encapsulation of Protocorm Like Bodies and in vitro regeneration of Asparagus racemosus Willd.

Vineet SONI 1, *
Preeti SHARMA 2

  1. Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur-313001, India
  2. School of Life Science, Jaipur National University, Jaipur-302025, India
Correspondence to: Vineet SONI, Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur-313001, India. Email: pvphuc@bmrat.org.
Volume & Issue: Vol. 3 No. 4 (2017) | Page No.: 77-79 |
Published: 2017-12-31

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Copyright © 2017 by the author(s).

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Various concentrations of sodium alginate and calcium chloride were tested in order to optimize the shape, and texture of synthetic seeds developed through the encapsulation of Protocorm Like Bodies (PLBs) of Asparagus racemosus. Sodium alginate (2.5%) and CaCl2.2H2O (100 mM) solutions were found to be the optimal concentration for production of firm, transparent and uniform synthetic seeds. The beads showed 94.9% germination on MS basal medium within three weeks. The germinated seedlings formed complete plantlet having well developed root-shoot system. The developed protocol for synthetic seed production through PLBs can be used for conservation of A. racemosus.

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