Open Access and Free to Publish in Biotechnological Research

Biotechnological Research Logo

Biotechnological Research

An open-access journal published by BioMedPress
Research articles Open Access Logo

Isolation and detection of CAMv5S promoter and cry gene from the genetically modified cotton varieties

Vinoth KANNA A 1, 2, *
Loyola PRINCY E 1

  1. Department of Biotechnology, Thanthai Hans Roever College, Elambalur, Perambalur – 621 212, Tamil Nadu, India
  2. Department of Molecular Biology, School of Life Sciences, Madurai Kamaraj University, Madurai – 625 017, Tamil Nadu, India
Correspondence to: Vinoth KANNA A, Department of Biotechnology, Thanthai Hans Roever College, Elambalur, Perambalur – 621 212, Tamil Nadu, India; Department of Molecular Biology, School of Life Sciences, Madurai Kamaraj University, Madurai – 625 017, Tamil Nadu, India. Email: pvphuc@bmrat.org.
Volume & Issue: Vol. 2 No. 1 (2016) | Page No.: 1-5 |
Published: 2016-01-31

Online metrics

Statistics from the website

  • HTML Views: 996
  • PDF Views: 452

Statistics from Dimensions

Statistics from PlumX
Copyright © 2016 by the author(s).

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Cotton (Gossypium arboretum) plays an important role in the day and daily life of humans. The production of cotton has been affected by various number of insect pest causes a serious infestation and infection which leads to vast damage to the cotton and its producers. In this study, the pre hybrid varieties such as MECH-184 and RCH 2 have been taken which was compared with a non Bt cotton variety (as a control) were studied for the presence of CaMV 35S promoter and cry genes. This has been studied using the PCR and lateral flow methods. From the results, these hybrid varieties have contained both the CaMV 35S promoter and cry genes.

Comments