Open Access and Free to Publish in Biotechnological Research

Research articles Open Access Logo

An efficient in vitro method for mass propagation of Plumbago zeylanica L. through nodal explants

Vineet SONI 1, *
Ira SHARMA 2
  1. Department of Botany, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, India
  2. School of Life Science, Jaipur National University, Jaipur-302025, Rajasthan, India
Correspondence to: Vineet SONI, Department of Botany, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, India. Email: pvphuc@bmrat.org.
Volume & Issue: Vol. 1 No. 2 (2015) | Page No.: 135-138 |
Published: 2015-06-30

Online metrics


Statistics from the website

  • HTML Views: 1232
  • PDF Views: 757

Statistics from Dimensions

Statistics from PlumX

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

A rapid and efficient protocol for the large-scale propagation of a potential medicinal plant, Plumbago zeylanica L., through in vitro culture of nodal explants is described. High frequency multiple shoots were induced on Murashige and Skoog's basal medium supplemented with 3.0 mg/l kinetin (KIN) and 0.5 mg/l 6-benzyl amino purine (BAP). The in vitro developed shoots were rooted best on half-strength MS medium fortified with 1.0 mg/l indole-3-acetic acid (IAA) and 0.5 mg/l 1-naphtalene acetic acid (NAA). The developed micropropagation protocol can be successfully used for large-scale multiplication and conservation of P. zeylanica.

Comments