Optimization of process parameters for β- glucosidase production by Byssochlamys fulva in slurry state fermentation
- P.G. and Research Department of Botany. St. Peter’s College Kolenchery
- Centre for Biofuels, Biotechnology Division, NIIST, Trivandrum
- Department of Biotechnology, University of Calicut, Calicut
Abstract
Byssochlamys fulva showing glucose tolerant β-glucosidase production was used in the present study. The production of BGL and GBGL in slurry state fermentation using this strain was optimized using statistical methodology. To screen factors affecting BGL and GBGL production, an ‘Optimal Factorial Design’ was created. Of the four factors selected, wheat bran concentration and incubation days showed significant positive effect on both BGL and GBGL production. Maximum BGL production of 1300.98 U/ml was noticed with incubation days 8, pH 8, agitation-100 rpm and wheat bran concentration 30 % w/v. Wheat bran concentration and incubation days were selected for further optimization using Response Surface Methodology (Central Composite design). After Response Surface optimization, there was a 2.52 % increase in BGL production (from 1300.9 U/ml to 3281 U/ml) and 1.53 % increase in GBGL production ( from 231.6 U/ml to 355.1 U/ml).